Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.5.1.18 (glutathione S-transferase)
 22,582 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To establish an in vivo radiation carcinogenesis model using glutathione S-transferase placental form positive (GST-P+) hepatic foci, newborn rats were irradiated once by 0.5 Gy and 2 Gy of gamma ray or 0.15 Gy and 0.6 Gy of neutron with or without 0.05% phenobarbital (PB). When the rats were sacrificed at the 12th or 21st week, the incidence of GST-P+ foci induction by radiation alone was very low. The neutron was more sensitive than the gamma ray at week 12 and the reverse phenomenon was observed in the groups at week 21. PB combination showed an increased incidence of GST-P+ foci in gamma ray irradiated groups. The neutron irradiation combined with PB did not show any significant difference compared with the corresponding PB untreated groups. We also investigated the combined effect of diethylnitrosamine (DEN) and 0.75 Gy of gamma ray irradiation. Intraperitoneal injection of 0.15 mumol/g body weight of DEN at 1 hour after gamma ray irradiation showed significantly increased the number and area of GST-P+ foci compared with those of DEN alone or DEN at 1 hour before gamma radiation (P < 0.001). From these data, after more defined experiments, an in vivo radiation carcinogenesis model will be established by radiation alone or a combination of radiation and carcinogens.
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PMID:Development of altered hepatocyte foci by separate and combined treatments with radiation and diethylnitrosamine in neonatal rats. 784 79

Our previous data suggested that in Sprague-Dawley (SD) rats, a short-term in vivo radiation carcinogenesis model can be established using glutathione S-transferase placental form (GST-P) positive hepatic foci induced by diethylnitrosamine (DEN) after gamma radiation. In the present study, differences between species, strain and sexes in susceptibility to radiation or radiation combined with DEN were examined. Newborn Fischer 344 (F344) and SD rats, and N:GP(S) mice were irradiated with whole body single 3Gy of gamma rays with or without intraperitoneally-injected DEN (15 mg/kg body weight) within 1 h of irradiation. Three weeks later, 0.05% phenobarbital was given in drinking water as a promoter, and at week 21 after birth, all animals were sacrificed. In both the radiation alone and radiation combined with DEN groups, F344 rats were more susceptible than SD rats to the induction of GST-P+ foci. In F344 rats, no significant difference in foci induction was seen between the sexes in both radiation alone and radiation combined with DEN groups, but in SD rats, female rats were more susceptible than males. In the case of mice, lung tumor incidence was examined, and in the radiation alone group, no differences between the sexes were seen; in the radiation combined with DEN group, however, females were more susceptible than males. These results suggested that susceptibility to radiation carcinogenesis could be potentiated by chemical carcinogens and showed wide variation between species, strains and sexes, of experimental animals.
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PMID:Species, strain and sex differences in susceptibility to gamma radiation combined with diethylnitrosamine. 961 73