Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.5.1.18 (glutathione S-transferase)
 22,582 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In this study, the antiulcerogenic effect of a water extract obtained from the lichen species Usnea longissima was investigated using indomethacin-induced ulcer models in rats. Experimental groups consisted of six rats. Antiulcerogenic activities of 50, 100 and 200mg/kg body wt. doses of the water extract were determined by comparing the negative (treated only with indomethacin) and positive (ranitidine) control groups. Although all doses of the water extract of U. longissima showed significant antiulcerogenic activity as compared to negative control groups, the highest activity was observed with 100 mg/kg body wt. doses (79.8%). The water extract of U. longissima showed moderate antioxidant activity when compared with trolox and ascorbic acids used as positive antioxidants. In addition, the activities of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST)] were determined in the stomach tissues of rats and compared with those of the negative and positive control groups to expose the effects of antioxidant enzymes on antiulcerogenic activity. SOD and GST enzymes activities in indomethacin-administrated tissues were reduced significantly by indomethacin in comparison to control groups. These enzymes were activated, however, by the water extracts of U. longissima. In contrast to SOD and GST activities, CAT activity was increased by indomethacin and reduced by all doses of U. longissima and ranitidine. The present results indicate that the water extract of U. longissima has a protective effect in indomethacin-induced ulcers, which can be attributed to its antioxidant potential.
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PMID:Effects of water extract of Usnea longissima on antioxidant enzyme activity and mucosal damage caused by indomethacin in rats. 1619 53

This work was undertaken to explore the potential of proteomics to dissect parallel and consecutive events of cadmium stress response in the lichen Physcia adscendens (Fr.) H. Olivier. Thalli were exposed to 0 (control) and 36 microM Cd for 6, 18, 24 and 48 h. Two-dimensional electrophoresis and mass spectrometry analyses showed an 80-85% spot identity between 6 and 18 h vs. 24 and 48 h of Cd exposure. Putative heat-shock proteins and glutathione S-transferase generally increased their expression all over the Cd treatments. By contrast, ABC transporters were underexpressed after 6-18 h, but in some cases induced after 24-48 h of Cd exposure. The cytochrome P450 appeared to have a variable expression pattern over time. Overall these data suggest that a considerable importance in the response of P. adscendens thalli to Cd stress can be assumed by differential expression of various protein families.
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PMID:Proteomic analysis in the lichen Physcia adscendens exposed to cadmium stress. 1851 71

Determining the origins of heavy metals, their accumulation, and their detoxification mechanisms constitutes a major problem in understanding environmental pollution in urban areas. The objective of this study was to detect the relative air quality in Eskisehir city center (Turkey) through the transplanted epiphytic lichen Pseudovernia furfuracea as a biomonitor of the concentrations of some heavy metals accumulated and to describe their toxic effects on lichen physiology during the study period of one year. The influence of heavy metal accumulations on macroelement levels were also examined. In addition to analysis of B, K, Ca, P, S, Al, Fe, Mg, Mn, Ni, Zn, Pb, and Cu, total soluble proteins and oxidative stress parameters through glutathione (GSH) contents and glutathione S-transferase (GST) activities were also determined. Results indicate that, although the heavy metal concentrations were found to be gradually increased from the unpolluted control zone to the city center, the concentrations of macroelements S and Ca were higher in the polluted area. However, B, K, Mg, and P elements were found to be higher in the unpolluted area than in the polluted zones. It seems that heavy metals induce oxidative stress in lichens; we found enhancement of GSH concentrations and GST activity. Most probably, the expected destruction in this organism was reduced by the intervention of antioxidant capacity. Therefore, this report confirms the fact that the epiphytic lichen P. furfuracea is a good model for biomonitoring atmospheric quality for a long-term transplantation study, and oxidative stress parameters stand out as a tool for an early environment assessment of other physiological parameters.
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PMID:Effects of urban air pollutants on elemental accumulation and identification of oxidative stress biomarkers in the transplanted lichen Pseudovernia furfuracea. 2146 37