Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.99.6 (
sialyltransferase
)
1,546
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We previously cloned and characterized the promoter region of the human NeuAcalpha2,3Galbeta1,3GalNAcalpha2,6-
sialyltransferase
(hST6GalNAc IV) gene [Kim et al. (2003)]. In the present study, we identified a region of 294 bp upstream of exon 1 of the gene that produced maximal transcriptional activity in human Jurkat T cells. Site-directed mutagenesis and transient transfection assays demonstrated that Sp1 and
MZF1
elements in this region were required for the promoter activity. Further analysis by electrophoretic mobility shift assays using specific competitors and antibody revealed that Sp1 and
MZF1
nuclear proteins interacted with these elements. These results indicate that Sp1 and
MZF1
are involved in the transcriptional regulation of the hST6GalNAc IV gene in Jurkat T cells.
...
PMID:Regulatory elements involved in transcription of the human NeuAcalpha2,3Galbeta1,3GalNAcalpha2,6-sialyltransferase (hST6GalNAc IV) gene. 1552 90
