Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.4.99.6 (sialyltransferase)
 1,546 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

There is a marked increase in sialyltransferase activity (EC 2.4.99.1) in serum and a profound change in the endogenous acceptor property of sialyltransferase in the intestine of colchicine treated rats (Fraser, Ratnam, Collins and Mookerjea, (1980) J. Biol. Chem. 255, 6617-6625). To ascertain the contribution of intestine as a source of this elevated serum enzyme, sialyltransferase and other enzymes activities were measured in intestinal lymph before and after colchicine treatment. There was a 4-fold increase of the enzyme activity in lymph 3 h after treatment. The lymph flow rate, protein concentration and composition as measured by polyacrylamide gel electrophoresis were not affected. The kinetic properties of lymph sialyltransferase (protein and time dependence, pH optima and Km values for the substrate CMP-sialic acid) were essentially unchanged after treatment and were similar to the serum sialyltransferase. Alkaline phosphatase and lactic dehydrogenase activities remained unchanged. Although intestinal lymph sialyltransferase was increased by colchicine, enterectomy did not prevent the rise of serum sialyltransferase suggesting that the intestine is not a major source of the serum enzyme.
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PMID:Elevated sialyltransferase activity in the intestinal lymph of colchicine-treated rats. 722 25

Sodium butyrate and dimethylsulfoxide (DMSO) have marked effects on the growth, morphology, and biochemistry of two human colonic adenocarcinoma cell lines in culture. Doubling times were increased between 18% and 660% while cell viability was unaffected. Both cell lines formed colonies in soft agar in the absence of butyrate of DMSO, but no colonies were observed in the presence of these agents. However, no differences in in vivo tumorigenicities, when cells were implanted in athymic mice, were seen following treatment. Gross morphological alterations including cell enlargement, process formation, and cellular flattening occurred during culture in butyrate or DMSO. Acrylamide gel electrophoresis in sodium dodecyl sulfate revealed no change in membrane protein constituents, but autoradiographic analysis of membrane glycoproteins demonstrated differences between treated and untreated cells. Ganglioside compositions were altered, and a sialyltransferase required for the synthesis of GM3 ganglioside was elevated by butyrate. Although cytoplasmic aminooligopeptidase remained unaffected by butyrate or DMSO, brush border-associated activity was enhanced by butyrate. Alkaline phosphatase also rose dramiatically during culture in butyrate but was not enhanced by DMSO.
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PMID:Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon cancer cells. 735 11