Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.99.6 (
sialyltransferase
)
1,546
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Campylobacter jejuni
causes more than 2 million cases of gastroenteritis annually in the United States, and is also linked to the autoimmune sequelae Guillan-Barre syndrome (GBS). GBS often results in flaccid paralysis, as the myelin sheaths of nerve cells are degraded by the adaptive immune response. Certain strains of
C. jejuni
modify their lipooligosaccharide (LOS) with the addition of neuraminic acid, resulting in LOS moieties that are structurally similar to gangliosides present on nerve cells. This can trigger GBS in a susceptible host, as antibodies generated against
C. jejuni
can cross-react with gangliosides, leading to demyelination of nerves and a loss of signal transduction. The goal of this study was to develop a quantitative PCR (qPCR) method and use whole genome sequencing data to detect the
Campylobacter
sialyltransferase
(
cst
) genes responsible for the addition of neuraminic acid to LOS. The qPCR method was used to screen a library of 89
C. jejuni
field samples collected by the Food and Drug Administration Pacific Northwest Lab (PNL) as well as clinical isolates transferred to PNL.
In silico
analysis was used to screen 827
C. jejuni
genomes in the FDA GenomeTrakr
SRA
database. The results indicate that a majority of
C. jejuni
strains could produce LOS with ganglioside mimicry, as 43.8% of PNL isolates and 46.9% of the GenomeTrakr isolates lacked the
cst
genes. The methods described in this study can be used by public health laboratories to rapidly determine whether a
C. jejuni
isolate has the potential to induce GBS. Based on these results, a majority of
C. jejuni
in the PNL collection and submitted to GenomeTrakr have the potential to produce LOS that mimics human gangliosides.
...
PMID:Whole Genome Sequencing and Multiplex qPCR Methods to Identify
Campylobacter jejuni
Encoding
cst-II
or
cst-III
Sialyltransferase. 2961 86
