Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.99.10 (
sialyltransferase
)
1,547
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
SAT-3
activity (CMP-NeuAc:Gal beta 1-4GlcNAc beta 1-3 Gal beta 1-4Glc-ceramide alpha 2-3 sialytransferase) involved in the biosynthesis of sialy Le(x) has been characterized in human colon carcinoma cells and embryonic chicken brains. Using RT-PCR-based strategy, we have isolated partial cDNA clones of
SAT-3
from ECB and Colo-205 mRNAs. Suitable primers from sialylmotif and N-terminal sequence of human placenta
SAT-3
(HP-SAT-3) were used. The 800 bp cDNA fragment encoding a region (90%) of alpha 2-3
sialyltransferase
(
SAT-3
) catalytic domain from ECB has been expressed as a
glutathione S-transferase
(
GST
) soluble fusion protein (62 kDa) in E. coli and purified over glutathione-agarose affinity matrix. Polyclonal antibody has been produced against affinity-purified catalytic domain of
SAT-3
(
GST
-SAT-3 fusion protein). A concentration-dependent polydonal antibody binding to native
SAT-3
has also been demonstrated by measuring the residual
SAT-3
activity in the enzyme fractions from Colo-205. The marked inhibition (> 80%) of
SAT-3
activity and relatively less inhibition (< 20%) of SAT-4 activity (CMP-NeuAc:GgOse4Cer alpha 2-3sialyl transferase) suggests strongly the existence of two different gene products (
SAT-3
and SAT-4) in human colon carcinoma Colo-205 cells and in embryonic chicken brains (ECB).
...
PMID:Cloning and expression of SAT-3 involved in SA-Le(x) biosynthesis: inhibition studies with polyclonal antibody against GST-SAT-3 fusion protein. 934 36
