Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.4.2.8 (hypoxanthine-guanine phosphoribosyltransferase)
 2,527 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The induction of gene mutations and chromosome aberrations by plasmid pEJ6.6 carrying the activated c-Ha-ras-1 oncogene from human bladder carcinoma was studied in cultured Chinese hamster cells. Both an increase in the frequency of hypoxanthine-phosphoribosyltransferase-deficient (HPRT-) mutants and chromosome aberrations was observed after pEJ6.6 transfection as compared to control series (pBR322). In order to define whether it is the oncogene which is responsible for the mutagenic effect of pEJ6.6, a derivative of c-Ha-ras-1 carrying a deletion in its coding region was constructed. As shown in all experiments, the frequency of HPRT- mutants after treatment with pEJ6.6 plasmid exceeded that in control dishes treated by pEJ6.6 plasmid with an inactivated oncogene. The effect was rather weak but statistically significant. Thus, the results of experiments carried out show that the mutagenic activity of pEJ6.6 plasmid is chiefly determined by its oncogene. The role of the mutagenic effects of activated oncogenes in malignant transformation is discussed.
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PMID:The activated human c-Ha-ras-1 oncogene as a mutagen. 152 Dec 28

The methylation of three human genes containing CpG islands and a CpG-depleted gene were measured in sperm, fetal and adult tissues. The c-Ha-ras was methylated extensively in the 3' region in sperm with a methylation-free region extending from the promoter to the third exon. The extent of methylation in the 3' region decreased in fetal cells, however, de novo methylation of sites closer to the island and within exon 1 were apparent. These sites were more completely methylated in adult lymphocytes and kidney. Essentially similar results were obtained with the CpG-island-containing genes, c-myc and HPRT (encoding hypoxanthine phosphoribosyl transferase), which showed that unmethylated sites near the CpG islands in sperm became methylated in fetal and adult cells. The variations in methylation seen in the non-island regions of the c-Ha-ras gene were mirrored in the insulin-encoding gene which does not contain a CpG island. The results show similar variations in methylation of non-island regions of DNA which occur independent of expression, and show that regions of extensive methylation in sperm may move closer to CpG islands in fetal and adult somatic cells.
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PMID:Methylation of CpG-island-containing genes in human sperm, fetal and adult tissues. 160 3

We have investigated two pedigrees in an attempt to detect the putative linkages between affective disorder and c-Ha-ras-1 oncogene and the insulin gene on chromosome 11, or hypoxanthine phosphoribosyltransferase (HPRT) on X chromosome. The linkage between affective disorders and the markers on chromosomes 11 and X was ruled out with the assumption of no recombination.
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PMID:Linkage analysis of affective disorder using DNA markers on chromosomes 11 and X. 175 90