Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.8 (
hypoxanthine-guanine phosphoribosyltransferase
)
2,527
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A new selective medium has been developed for cells containing the enzyme
deoxycytidine deaminase
. This medium contains hypoxanthine, aminopterin, and 5-methyldeoxycytidine (HAM medium). To survive in the presence of the aminopterin, the cells must utilize
deoxycytidine deaminase
to convert the 5-methyldeoxycytidine to thymidine. The cells must also have thymidine kinase and
hypoxanthine phosphoribosyltransferase
. A mouse cell line deficient in
deoxycytidine deaminase
has been isolated from a deoxycytidine kinase-deficient line, using 5-bromodeoxycytidine as the selective agent. A hybrid line between this double mutant and a human diploid fibroblast was isolated in HAM medium. The hybrid line contains the chromosomes expected of a human-mouse hybrid. The
deoxycytidine deaminase
isozyme patterns on cellogel show that the human-mouse hybrid cell line produces an enzyme with an electrophoretic mobility intermediate between that of the human and that of the mouse.
...
PMID:A human-mouse somatic hybrid line selected for human deoxycytidine deaminase. 123 1
