Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.4.2.8 (hypoxanthine-guanine phosphoribosyltransferase)
 2,527 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The karyotype of microcebus murinus (MIM) (lemuridae) is considered by Dutrillaux (1979) as the closest to the karyotype ancestral to all primates. A large number of homoeologies exists between the banding patterns of MIM chromosomes and those of man (HSA). We report a comparison of the gene maps of these two species which confirms most of these homoeologies. Fifteen cell hybrids were obtained by fusing MIM fibroblasts and an HPRT- Chinese hamster cell line. Twenty-seven enzyme markers were investigated. The following assignments were demonstrated: NP to chromosome MIM 2, homoeologous to HSA 14; the syntenic group PGD-ENO1-PGM1 to MIM 3, homoeologous to HSA 1p; LDHA to MIM 5, homoeologous to HSA 11; Me1 to MIM 6, homoeologous to HSA 6; the syntenic group LDHB-CS-PEPB-ENO2-TPI to MIM 7, homoeologous to HSA 12; the syntenic group AK1-AK3 to MIM 10, which we considered to be homoeologous to HSA 9 (we do not consider MIM 9 to be homoeologous to HSA 9, as does Dutrillaux, 1979); GOT1 to MIM 15, homoeologous to HSA 10; the syntenic group HPRT-G6PD-PGK-GLA to MIM X. Synteny dissociation in three hybrids suggests closer linkage between G6PD and HPRT than between PGK-GLA and HPRT. Three syntenic groups, known in man, were confirmed in MIM but could not be assigned with full confidence: ACP1-MDH1, MP1-PKM2, and PEPD-GPI. GUK1 and PEPC, known to be syntenic in man, were found to be asyntenic in MIM and could not be assigned. PGM2 and SOD1 could not be assigned. A comparison of these gene assignments with those known in Cebus capucinus showed a remarkable homoeology for six chromosomes of the two species.
 ...
PMID:Gene mapping of Microcebus murinus (Lemuridae): a comparison with man and Cebus capucinus (Cebidae). 695 81

To investigate bystander mutagenic effects induced by alpha particles during boron neutron capture therapy (BNCT), we mixed cells that were electroporated with borocaptate sodium (BSH), which led to the accumulation of (10)B inside the cells, with cells that did not contain the boron compound. BSH-containing cells were irradiated with alpha particles produced by the (10)B(n,alpha)(7)Li reaction, whereas cells without boron were only affected by the (1)H(n,gamma)(2)H and (14)N(n,rho)(14)C reactions. The frequency of mutations induced in the hypoxanthine-guanine phosphoribosyltransferase (HPRT) locus was examined in Chinese hamster ovary (CHO) cells irradiated with neutrons (Kyoto University Research Reactor: 5 MW). Neutron irradiation of 1:1 mixtures of cells with and without BSH resulted in a survival fraction of 0.1, and the cells that did not contain BSH made up 99.4% of the surviving cell population. Using multiplex polymerase chain reactions (PCRs), molecular structural analysis indicated that most of the mutations induced by the bystander effect were point mutations and that the frequencies of total and partial deletions induced by the bystander effect were lower than those resulting from the alpha particles produced by the (10)B(n,alpha)(7)Li reaction or the neutron beam from the (1)H(n,gamma)(2)H and (14)N(n,rho)(14)C reactions. The types of point mutations induced by the BNCT bystander effect were analyzed by cloning and sequencing methods. These mutations were comprised of 65.5% base substitutions, 27.5% deletions, and 7.0% insertions. Sequence analysis of base substitutions showed that transversions and transitions occurred in 64.7% and 35.3% of cases, respectively. G:C-->T:A transversion induced by 8-oxo-guanine in DNA occurred in 5.9% of base substitution mutants in the BNCT bystander group. The characteristic mutations seen in this group, induced by BNCT alpha particles, differed from those typical of gamma ray radiation.
 ...
PMID:Bystander effect-induced mutagenicity in HPRT locus of CHO cells following BNCT neutron irradiation: characteristics of point mutations by sequence analysis. 1937 34