Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.8 (
hypoxanthine-guanine phosphoribosyltransferase
)
2,527
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The enzyme
hypoxanthine-guanine phosphoribosyltransferase
(
HGPRT
) of Plasmodium falciparum plays a key role in the salvage of preformed purine nucleotides from parasite-infected erythrocytes. Since P. falciparum cannot synthesize purines de novo, development of inhibitors specific for the parasite
HGPRT
should be an effective method of chemotherapy. To provide sufficient amounts of
HGPRT
for biochemical and crystallographic analysis, we have isolated the P. falciparum
HPRT
cDNA sequence and expressed it in an Escherichia coli strain deficient for both de novo purine synthesis and guanine utilization (strain
GP120
).
GP120
cells containing the P. falciparum
HPRT
plasmid vector (pRD500), when grown in the presence of isopropyl-beta-D-thiogalactopyranoside (IPTG) which induces the tac promoter of the expression vector, produce a novel protein of 26 kDa, which is in agreement with the predicted Mr deduced from the
HPRT
cDNA open reading frame. In addition, we have demonstrated significant
HGPRT
activity in cell-free extracts of
GP120
[pRD500] cultures grown in minimal medium containing xanthine, as the sole source of purines, and IPTG.
...
PMID:Cloning and expression in Escherichia coli of a hypoxanthine-guanine phosphoribosyltransferase-encoding cDNA from Plasmodium falciparum. 220 41
