Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.7 (
adenine phosphoribosyltransferase
)
692
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A method is described for obtaining antibody-producing hybridomas that are preferentially retained in cultures of fused mouse spleen and myeloma cells. Hybridomas are produced by fusing mouse myeloma cells that are deficient in
adenosine phosphoribosyltransferase
(
APRT
) with mouse spleen cells containing Robertsonian 8.12 translocation chromosomes. The cell fusion mixtures are exposed to a culture medium that can be utilized only by
APRT
-positive cells, which results in the elimination of both unfused
APRT
-deficient myeloma cells and non-antibody-producing
APRT
-deficient hybridomas that arise by segregation of the 8.12 translocation chromosomes containing the
APRT
genes and the active
heavy chain
immunoglobulin gene.
...
PMID:Stable antibody-producing murine hybridomas. 640 15
