Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.4.2.7 (adenine phosphoribosyltransferase)
 692 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Twenty-three silver fox-Chinese hamster somatic cell hybrids were analysed for the expression of fox enzyme loci and the segregation of fox chromosomes. This analysis made it possible to assign the gene PGD to chromosome 2, MDH2 to chromosome 3. NP to chromosome 10. APRT, ENO1, PGM1 to chromosome 12, MDH1 and IDH1 to chromosome 16. Possible use of the above-mentioned clone panel for fox gene mapping is analysed. An attempt to reveal homologous regions on fox and human chromosomes was made by comparative analysis of prometaphase fox and human chromosomes containing the homologous genes. The means and perspectives of verification of the hypothesis proposed are discussed.
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PMID:[Genome mapping of the silver fox. I. Determination of the chromosomal location of 8 fox genes and the search for homologous regions on fox and human chromosomes]. 316 22

Twenty-three silver fox x hamster somatic cell hybrid clones were used to assign 15 fox genes: GPI to chromosome 1; PGD to chromosome 2; MDH2 to chromosome 3; ESD to chromosome 6; LDHB to chromosome 8; NP to chromosome 10; LDHA to chromosome 11; APRT, ENO1, and PGM1 to chromosome 12; IDH1 and MDH1 to chromosome 16; and GLA, G6PD, and HPRT to the X chromosome. High-resolution G-banding of human, cat, mink, and fox chromosomes containing homologous regions (according to genetic maps) revealed regions of putative homology. The results lend support to the suggestion that the most considerable karyotypic reorganization of the ancestral genome in the order Carnivora occurred during Canidae formation. The details of karyotypic evolution in mammals are discussed.
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PMID:Silver fox gene mapping: conserved chromosome regions in the order Carnivora. 319 55

Isolation of electrophoretic mobility shift mutants for a large number of enzyme loci in CHO cells has allowed the identification of many genes which are functionally hemizygous. To gain further insight into the nature of hemizygosity in CHO cells and the mechanisms by which it has arisen, we are attempting to determine whether hemizygous gene loci are clustered in a few localized chromosomal regions in CHO or are more generally distributed throughout the genome. Isozyme analysis of a series of CHO electrophoretic mobility shift mutants for MDH2 (malate dehydrogenase 2, EC 1.1.1.37) revealed that this locus is functionally hemizygous in CHO cells, but the locus could not be mapped by conventional approaches because of the similar electrophoretic mobilities of Chinese hamster and mouse MDH2 isozymes. Construction of intraspecific CHO X CHO hybrids using electrophoretic mobility shift mutants with secondary, selectable drug-resistance markers allowed us to determine that MDH2 is not closely linked to any previously mapped hemizygous marker loci in CHO, but is linked to alleles for two dizygous gene loci, PGM3 and APRT, on CHO chromosome Z7. A possible genetic basis for hemizygosity of the MDH2 locus in CHO cells is discussed.
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PMID:Functional hemizygosity for the MDH2 locus in Chinese hamster ovary cells. 345 74