Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.4.2.7 (adenine phosphoribosyltransferase)
 692 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A methodology is presented for systemic analysis of purine enzymes in small lymphocyte subfractions. For the determination of 7 different enzymes of purine metabolism *hypoxanthine-guanine phosphoribosyltransferase (HG-PRT), adenine phosphoribosyltransferase (A-PRT), adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), adenosine kinase (AK), 5'-nucleotidase (5'N), and AMP-deaminase) less than 200,000 peripheral blood lymphocytes are needed. 1000-6000 lyophilised lymphocytes are incubated in micro-incubation vessels (3 microliter) with radioactive substrates for 15-180 min. Separation of substrates and products is achieved by thin-layer chromatography on PEI-cellulose. Addition of BSA to the incubation mixtures results in higher specific enzyme activities and narrower ranges of mean values of a control group.
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PMID:Enzymes of purine nucleotide metabolism in human lymphocytes. 625 89

In rat embryo cell cultures infected with X14 or H-1 parvovirus and in mock-infected cell cultures the activity of some enzymes involved in purine nucleotide interconversion and in "adenylate cycle" was determined. The enzymatic activities have been assayed on 100,000 x g supernatant by spectrophotometric methods, measuring the absorbance variation in U.V. and by radiometric methods, resolving the radioactive products of reaction by TLC on PEI cellulose. The results indicated a decrease of the enzymatic activities that degrade purine nucleosides and nucleotides in infected cells compared to the controls. Some different behaviour patterns showed the enzymes involved in base salvage pathway; adenine phosphoribosyltransferase did not show a significant modification of activity, whereas hypoxanthine (guanine) phosphoribosyltransferase increased slightly in X14 virus-infected cells. The behaviour of the above mentioned enzymatic activities may be considered as a mechanism of purine nucleotide saving, coupled to an active salvage pathway for the synthesis of nucleotides required for the viral replication.
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PMID:Activity of some enzymes involved in "adenylate cycle" in rat embryo cells infected with parvoviruses (X14, H-1). 732 10