Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.7 (
adenine phosphoribosyltransferase
)
692
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Changes in hepatic purine enzyme activities of chicks fed diets containing 11%, 20%, 43% and 80% protein were correlated with protein intake and uric acid production in order to identify those enzymes with activities that parallel closely and may regulate uric acid production. Nucleoside phosphorylase, xanthine dehydrogenase,
adenylosuccinate synthetase
and adenosine kinase correlated positively with protein intake and uric acid production. Adenosine deaminase, 5'-nucleotidase (AMP), adenylate deaminase and
adenine phosphoribosyltransferase
correlated negatively with protein intake and uric acid production. Hypoxanthine phosphoribosyltransferase and 5'-nucleotidase (IMP) were unaffected by protein intake and did not correlate with uric acid production. The ratio of adenosine kinase to adenosine deaminase correlated positively with protein intake and uric acid production. The increased activities of
adenylosuccinate synthetase
and adenosine kinase, along with the reduced activities of 5'-nucleotidase and adenylate deaminase, in liver from chickens fed the 80% compared with the 11% protein diet demonstrate enhanced synthesis of adenine nucleotides. Since adenine nucleotides are essential cofactors for de novo purine synthesis, it is proposed that
adenylosuccinate synthetase
, adenosine kinase, 5'-nucleotidase and adenylate deaminase are key enzymes involved in the regulation of purine biosynthesis.
...
PMID:Protein intake, hepatic purine enzyme levels and uric acid production in growing chicks. 61 42
Allopurinol was shown to be effective in vitro against Leishmania mexicana and Leishmania donovani as well as against Leishmania braziliensis. The major metabolic derivative of allopurinol in humans, oxipurinol, also is antileishmanial for L. donovani. The antileishmanial effect of allopurinol and oxipurinol can be specifically reversed by adenine, and its metabolic precursors and derivatives, but by no other purines or their derivative. It is proposed that the
adenylosuccinate synthetase
or the
adenine phosphoribosyltransferase
may be sites of action for these agents.
...
PMID:Antileishmanial effect of allopurinol. II. Relationship of adenine metabolism in Leishmania species to the action of allopurinol. 92 80
The enzymes that catalyse the salvage of purines in Entamoeba histolytica trophozoites have been surveyed. Adenine deaminase (EC 3.5.4.2), adenosine deaminase (EC 3.5.4.4), guanine deaminase (EC 3.5.4.3),
adenine phosphoribosyltransferase
(PRTase) (
EC 2.4.2.7
), xanthine PRTase (EC 2.4.2.22) and hypoxanthine PRTase (EC 2.4.2.8) were all detected in cell homogenates but only at low activities, whereas AMP deaminase (EC 3.5.4.6) and guanine PRTase (EC 2.4.2.8) were not found. Phosphorylases (EC 2.4.2.1) active in both anabolic and catabolic directions were present and all nucleosides tested were phosphorylated by kinases (EC 2.7.1.15, EC 2.7.1.20, EC 2.7.1.73). 3'-Nucleotidase (EC 3.1.3.6) and 5'-nucleotidase (EC 3.1.3.5) were found, the former being mainly particulate. Nucleotide interconversion enzymes (adenylosuccinate lyase, EC 4.3.2.2;
adenylosuccinate synthetase
,
EC 6.3.4.4
; IMP dehydrogenase, EC 1.2.1.14; GMP synthetase, EC 6.3.5.2 and GMP reductase, EC 1.6.6.8) were not detected. The results suggest that in E. histolytica the main route of nucleotide synthesis is from the individual bases through the actions of phosphorylases and kinases.
...
PMID:Purine-metabolising enzymes in Entamoeba histolytica. 287 91
The inhibition of Escherichia coli strain B and strain W-11 by 6-methylpurine depended on the formation of 6-methylpurine ribonucleotide by the action of
adenine phosphoribosyltransferase
(AMP: pyrophosphate phosphoribosyltransferase,
EC 2.4.2.7
). 6-Methylpurine ribonucleotide inhibited the de novo synthesis of purines, presumably via pseudofeedback inhibition of phosphoribosylpyrophosphate amidotransferase (EC 2.4.2.14). The same mechanism accounted for its inhibition of
adenylosuccinate synthetase
[IMP: l-aspartate ligase (GDP),
EC 6.3.4.4
]. Adenine and 6-methylaminopurine prevented inhibition by competing for the action of
adenine phosphoribosyltransferase
. In addition, adenine reversed this inhibition by replenishing the AMP to bypass both sites of inhibition. Nonproliferating suspensions of strain B-94, which lacked adenylosuccinate lyase (EC 4.3.2.2), converted exogenous hypoxanthine and aspartate to succinoadenine derivatives which accumulated in the medium. Compounds which inhibited
adenylosuccinate synthetase
inhibited accumulation of the succinoadenine derivatives. A method was described for the isolation of mutants which potentially possessed an altered
adenylosuccinate synthetase
.
...
PMID:Inhibition of de novo purine biosynthesis and interconversion by 6-methylpurine in Escherichia coli. 490 85
Most freshly isolated Trypanosoma cruzi blood trypomastigotes were insensitive to allopurinol (HPP) and 4-aminopyrazolo(3,4-d)pyrimidine (APP). Strains EP and Ya were, however, strongly inhibited by both drugs while strains DS and A-35 were HPP-insensitive but APP-sensitive. In contrast, epimastigotes resulting from one in vitro passage of all eleven T. cruzi strains were highly sensitive to both drugs. While hypoxanthine/guanine and
adenine phosphoribosyltransferase
and
succino-AMP synthetase
activities were similar in trypomastigotes of sensitive and insensitive T. cruzi strains, the uptake and metabolism of [14C]HPP and [14C]APP was significantly slower in T. cruzi trypomastigotes of insensitive strains than in sensitive strains. The results suggest the importance of drug uptake rates in determining the pyrazolopyrimidine sensitivity of different T. cruzi strains.
...
PMID:Differences in allopurinol and 4-aminopyrazolo(3,4-d) pyrimidine metabolism in drug-sensitive and insensitive strains of Trypanosoma cruzi. 637 52
The generation and characterization of new sheep-hamster cell hybrids is reported from the fusion of sheep white blood cells with six different hamster auxotrophs. Selection from these and previously generated cell hybrids has led to the production of a panel of 30 hybrids covering the complete sheep genome of 28 chromosomes. Over half of the cell hybrids in this panel contain single sheep chromosomes. By complementation, the following new assignments have been made using the panel: phosphoribosyl N-formylglycinamide amidotransferase (PRFGA) to sheep chromosome (chr) 11;
adenylosuccinate synthetase
(
ADSS
) to sheep chr 12; adenylosuccinate lyase (ADSL) to sheep chr 3q; 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGCS) to sheep chr 16; dihydrofolate reductase (DHFR) to sheep chr 5; and
adenine phosphoribosyltransferase
(
APRT
) to sheep chr 14. The gene phosphoribosylaminoinidazole-carboxamide formyltransferase/Inosinicase (PRACFT) has now been regionally assigned to chr 2q. By isozyme analysis, phosphogluconate dehydrogenase (PGD) was assigned to sheep chr 12, anchoring the sheep syntenic group U1 to this chromosome, and mannose phosphate isomerase (MPI) was assigned to sheep chr 18. Furthermore, the chromosomal assignment of 110 microsatellites was confirmed using this cell panel.
...
PMID:New gene assignments using a complete, characterized sheep-hamster somatic cell hybrid panel. 968 51
