Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.7 (
adenine phosphoribosyltransferase
)
692
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have used direct microinjection of messenger RNA into individual mouse and human cells to assay for specific translation products. We have been able to detect the synthesis of human fibroblast
interferon
, thymidine, kinase, hypoxanthine phosphoribosyltransferase,
adenine phosphoribosyltransferase
, and propionyl-CoA carboxylase in response to injected mRNA. Using the
interferon
system as a model, we have quantitated
interferon
synthesis and followed partial purification of
interferon
mRNA sequences on sucrose density gradients. The methods we have utilized should be applicable to other systems in which sensitive assays exist for gene products and should provide a screening procedure for isolating specific mRNA sequences.
...
PMID:Biological detection of specific mRNA molecules by microinjection. 29 82
