Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Non-SMC condensin I complex subunit H
(
NCAPH
) is a vital gene associated with chromosome stability and is required for proper chromosome condensation and segregation. However, the mechanisms through which
NCAPH
affects pancreatic cancer (PC) and its molecular function remain unclear. In this study, we examined the role of
NCAPH
in PC cells. Our results showed that
NCAPH
was overexpressed in clinical PC specimens (GEPIA) and cell lines. In addition, in
NCAPH
-knockdown cells, colony formation and proliferation were inhibited, and the cell cycle was arrested at the S and G
2
/M phases owing to failure of mature chromosome condensation (MCC) in poorly condensed chromosomes. Increased cell death in
NCAPH
-knockdown cells was found to help initiate apoptosis through the activation of caspase-3 and
PARP
cleavage. Furthermore,
NCAPH
-knockdown cells showed an increase in chromosomal aberrations and DNA damage via activation of the DNA damage response (Chk1/Chk2) signaling pathways. These data demonstrated that
NCAPH
played an important role in cell cycle progression and DNA damage by maintaining chromosomal stability through progression of MCC from poorly condensed chromosomes. Ultimately,
NCAPH
knockdown induced apoptotic cell death, which was partially mediated by caspase-dependent pathways. These findings highlight the potential role of
NCAPH
as a therapeutic target for PC.
...
PMID:Non-SMC condensin I complex subunit H mediates mature chromosome condensation and DNA damage in pancreatic cancer cells. 3178 46
