Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
G-protein-coupled receptors (GPCRs), the largest family of cell-surface molecules involved in a number of biological and pathological processes, have recently emerged as key players in carcinogenesis and cancer progression. Orphan G protein-coupled receptors (oGPCRs) are a group of proteins lacking endogenous ligands.
GPR137
, one of the novel oGPCR genes, was discovered by homology screening. However, the biological role of
GPR137
in cancers has not yet been discussed and is of great therapeutic interest. In this study, we knocked down
GPR137
via a lentivirus system in two human pancreatic cancer cell lines BXPC-3 and PANC-1. Knockdown of
GPR137
strongly inhibited cell proliferation and colony formation. Flow cytometry showed that cell cycle was arrested in the sub-G1 phase and apoptotic cells were significantly increased after
GPR137
knockdown. Western blotting confirmed that
GPR137
silencing induced apoptosis due to cleavage of
PARP
(poly ADP-ribose polymerase) and upregulation of caspase 3. Furthermore, lentivirus-mediated overexpression of
GPR137
promoted the proliferation of PANC-1 cells, suggesting
GPR137
as a potential oncogene in pancreatic cancer cells. Taken together, our results prove the importance of
GPR137
as a crucial regulator in controlling cancer cell growth and apoptosis.
...
PMID:Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis. 2547 90
