Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We screened a human lymphocyte cDNA library using the yeast two-hybrid system and an automodification domain of
PARP
as a probe. The DNA sequence of an isolated clone (clone 3-9) was identical to the partial cDNA sequence of the human
ribosomal protein S3a
. We confirmed that
PARP
interacts with clone 3-9 by performing binding studies using a GST-3-9 fusion protein as bait. We also demonstrated that native S3a in nuclear extracts of HL-60 cells interacts with the automodification domain of
PARP
and that
PARP
from nuclear extracts is coprecipitated with the GST-3-9 fusion protein. Furthermore, we demonstrated that Bcl-2 interacts with
PARP
in association with S3a and that the interaction of S3a and Bcl-2 with
PARP
causes a significant decrease in
PARP
activity. Since Bcl-2 failed to inhibit
PARP
activity in the absence of S3a, we suggest that Bcl-2 together with S3a prevents apoptosis probably by inhibiting
PARP
activity.
...
PMID:Inhibition of poly(ADP-ribose) polymerase activity by Bcl-2 in association with the ribosomal protein S3a. 1179 Jan 16
