Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The purpose of the article is to investigate the role of
IARS2
in proliferation, apoptosis, and cell cycle of gastric cancer (GC) cells in vitro. The
IARS2
-shRNA lentiviral vector was established and used to infect the GC cell line AGS. qRT-PCR and Western blot were employed to determine the efficiency of
IARS2
knockdown. The effects of
IARS2
knockdown on cell proliferation, cell clone formation, and cell cycle were assessed by MTT assay, colony formation assay, and flow cytometer analysis, respectively. Finally, a PathScan Antibody Array Kit was used to detect the expression levels of cell cycle-related proteins after
IARS2
knockdown in AGS cells to elucidate the underlying mechanisms. Compared with negative control group,
IARS2
was significantly knocked down by transfection with lentivirus encoding shRNA of
IARS2
in AGS cells.
IARS2
knockdown significantly inhibited the proliferation and colony formation ability and induced cycle arrest at G2/M phase of AGS cells.
IARS2
knockdown significantly decreased the expression levels of phosphorylation of (p-Smad2), p-SAPK/JUK, cleavage-Caspase-7, and p-TAK1, but increased the expression levels of p-53 and cleavage-
PARP
in AGS cells compared to shCtrl group. We demonstrated that
IARS2
knockdown inhibits proliferation, suppresses colony formation, and causes cell cycle arrest in AGS cells. We also found that
IARS2
regulates key molecules of cell apoptosis-related signaling pathway.
...
PMID:Knockdown of IARS2 suppressed growth of gastric cancer cells by regulating the phosphorylation of cell cycle-related proteins. 2907 39
