Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Nano silicon dioxide (nano-
SiO2
) is becoming more and more widely applied in the fields of industry. The potential toxic effects of nano-
SiO2
and its hazard to human health are drawing more attention. The mRNA expression of poly(ADP-ribose) polymerases-1(
PARP-1
), a pivotal repair gene, has been decreased by nano-
SiO2
exposure. However, the effect of epigenetic modification on nano-
SiO2
-induced low
PARP-1
expression has not been reported. In this study, HaCaT cells with or without DNA methyltransferase 1(DNMT1) knock down were incubated with nano-
SiO2
and then further treated with DNMT inhibitor, 5-aza-2-deoxycytidine (DAC), which is a kind of key epigenetic modification reagents. Real-time Q-PCR and western blotting were used to examine the mRNA and protein expression of
PARP-1
. For promoter methylation status of
PARP-1
, methylation-specific PCR (MSP) and Bisulfite sequencing assay were performed. Results showed a dramatic decrease of
PARP-1
expression on mRNA and protein level and a simultaneously obvious increase in the level of
PARP-1
methylation in nano-
SiO2
-treated cells compared to the control group. Further, the expression and promoter methylation of
PARP-1
in HaCaT cells were restored following DNMT1 knock down, suggesting that the effects of
PARP-1
promoter hypermethylation are mediated at least in part by DNMT1. Taken together, methylation of
PARP-1
promoter might be involved in the regulation of nano-
SiO2
-induced decrease of
PARP-1
expression.
...
PMID:Methylation of PARP-1 promoter involved in the regulation of nano-SiO2-induced decrease of PARP-1 mRNA expression. 2226 68
