Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Besides its well-recognized antiviral activity,
Cidofovir
(
CDV
) has been shown to exert anticancer properties both within in vitro and in vivo models. The aim of this study was to evaluate the effects of
CDV
on still unexplored cultured cancer cells from human mesothelioma as well as breast, colon, liver, lung, prostate, and thyroid carcinomas. Overall, a dose- and time-dependent inhibition of cell viability was observed after
CDV
exposure. To clarify the mechanisms underlying
CDV
action, apoptotic cell death was investigated in two infected cell lines [Ist-Mes1 and Ist-Mes2 mesothelioma cells (SV40+)] and in two uninfected cell lines (NCI-H2425 mesothelioma cells and FTC-133 thyroid cancer cells), which resulted the most sensitive to
CDV
treatment. Reduced expression of procaspase-3 and increased expression of
PARP
p85 fragment were observed in both infected and uninfected mesothelioma cells, indicating apoptosis induction by
CDV
in a virus-independent manner. Similarly, the increase of the pro-apoptotic proteins p53, cytochrome c and caspase-3, the decrease of the survival protein Bcl-x, and the increment of Bax/Bcl-2 ratio revealed the occurrence of apoptosis in
CDV
-treated FTC-133. The presence of nuclear DNA fragmentation confirmed apoptotic cell death by
CDV
. Overall, our findings warrant further investigations to explore the therapeutic potential of
CDV
for human mesothelioma and follicular thyroid carcinoma.
...
PMID:Reduced cell viability and apoptosis induction in human thyroid carcinoma and mesothelioma cells exposed to cidofovir. 2822 40
