Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The standard Pythium selective medium
PARP
(pimaricin + ampicillin + rifampicin + pentachloronitrobenzene [PCNB] agar), was modified by replacing PCNB and pimaricin with other antifungal agents. Several antifungal agents such as fluazinam, miconazole, 2,4,5,6-tetrachloroisophthalonitrile (TPN), iminoctadine triacetate, tolclofos-methyl, captan, and nystatin, initially were screened for effects on Pythium growth. Based on these results, the following three media were developed: PARF (pimaricin + ampicillin + rifampicin + fluazinam agar),
NARF
(nystatin + ampicillin + rifampicin + fluazinam agar), and NARM (nystatin + ampicillin + rifampicin + miconazole agar). New media were comparable with
PARP
on yield of naturally occurring Pythium spp. from two different types of soil using the soil-dilution plating technique. PARF and
NARF
were significantly better than
PARP
on inhibition of non-pythiaceous microbes on the soil-dilution plates, but were significantly lower than
PARP
on the rate of mycelial growth of six of eight isolates belonging to seven species of Pythium. NARM was equivalent to
PARP
on inhibition of non-pythiaceous microbes except for Fusarium oxysporum, and was significantly better than
PARP
on rate of mycelial growth of five of eight isolates of Pythium.
...
PMID:Modifications of PARP Medium Using Fluazinam, Miconazole, and Nystatin for Detection of Pythium spp. in Soil. 3078 May 96
