Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
SCIN
(scinderin) is a calcium-dependent actin severing and capping protein. Homologue in zebrafish has been found to be related with cell death. In the present study, we found that
SCIN
is highly expressed in human lung cancer specimens. However, the role of
SCIN
in lung cancer has not yet been determined. To investigate the function of
SCIN
in lung carcinoma cells, we took advantage of lentivirus-mediated RNA interference (RNAi) to knockdown
SCIN
expression in two lung carcinoma cell lines A549 and H1299. Silencing of
SCIN
significantly inhibited the proliferation and colony formation ability of both cell lines in vitro. Moreover, flow cytometry analysis showed that knockdown of
SCIN
led to G0/G1 phase cell cycle arrest as well as an excess accumulation of cells in the sub-G1 phase. Furthermore, depletion of
SCIN
resulted in a significant increase in Cyclin B1, p21 and
PARP
expression, and a little decrease in Cyclin D1 expression. These results suggest that
SCIN
plays an important role in lung carcinoma cell proliferation, and lentivirus-mediated silencing of
SCIN
might be a potential therapeutic approach for the treatment of lung cancer.
...
PMID:Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells. 2530 73
