Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:2.4.2.30 (
PARP
)
13,611
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Protocadherin-10
(
PCDH10
), a member of non-clustered protocadherin family which plays important roles in calcium-dependent cell-cell signal transduction and adhesion.
PCDH10
functions as a tumor suppressor gene and its expression is downregulated by promoter methylation in many malignances. In the present study, we explored
PCDH10
expression and promoter methylation status, and its biological effects in pancreatic cancer cells, and furthermore, we explored the mechanism of
PCDH10
preliminary in pancreatic cancer cells. the mRNA level of
PCDH10
was detected by semi-quantitative reverse transcription PCR and promoter methylation status examined by methylation-specific PCR in the pancreatic cancer cells (Capan-1, Panc-1, AsPC-1 and BxPC-3) as well as the human normal pancreatic ductal epithelial cells HPDE6-C7 which was used as a control. The human pancreatic cells were transfected with plasmid pcDNA3.1-
PCDH10
or pcDNA3.1 by lipofectamine 2000. The biological function of
PCDH10
in pancreatic cancer cells was determined by CCK-8 assay, colony formation assay, flow cytometry, Transwell invasion assay and wound-healing assay. The levels of apoptosis related proteins were examined by western blotting.
PCDH10
expression was obviously downregulated in the pancreatic cancer cells (Capan-1, Panc-1, BxPC-3) compared to the normal pancreatic ductal epithelial cells.
PCDH10
promoter methylation was observed in the two pancreatic cancer cells Capan-1 and BxPC-3,and the expression of
PCDH10
could be restored after treating with 5-aza-2'-deoxycytidine and trichostatin A in the two cell types. Overexpression of
PCDH10
can inhibit the proliferation, migration, invasion ability of pancreatic cancer cells and induce apoptosis. Ectopic expression of
PCDH10
could increase the levels of
PARP
, caspase-3, caspase-9 and decrease the level of bcl-2, AKT and p-AKT.
PCDH10
acts as a tumor suppressor gene, and is frequently down-regulated by promoter methylation in pancreatic cancer cells.
PCDH10
may induce cancer cell apoptosis via the AKT pathway.
...
PMID:Protocadherin-10 acts as a tumor suppressor gene, and is frequently downregulated by promoter methylation in pancreatic cancer cells. 2717 20
