Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.1.18 (
branching enzyme
)
628
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Starch
branching enzyme
(SBE) has rarely been studied in common starchy banana fruits. For the first time, we report here the molecular characterization of seven
SBE
(
MaSBE
) and six
SBE
(
MbSBE
) genes in the banana A- and B-genomes, respectively, which could be classified into three distinct subfamilies according to genome-wide identification. Systematic transcriptomic analysis revealed that six
MaSBE
s and six
MbSBE
s were expressed in the developing banana fruits of two different genotypes, BaXi Jiao (BX,
AAA
) and Fen Jiao (FJ, AAB), among which
MaSBE2.3
and
MbSBE2.3
were highly expressed. Transient silencing of
MaSBE2.3
expression in banana fruit discs led to a significant decrease in its transcription, which coincides with significant reductions in total starch and amylopectin contents compared to those of empty vector controls. The suggested functional role of
MaSBE2.3
in banana fruit development was corroborated by its transient overexpression in banana fruit discs, which led to significant enhancements in total starch and amylopectin contents. A number of transcription factors, including three auxin response factors (ARF2/12/24) and two MYBs (MYB3/308), that interact with the
MaSBE2.3
promoter were identified by yeast one-hybrid library assays. Among these ARFs and MYBs, MaARF2/MaMYB308 and MaARF12/MaARF24/MaMYB3 were demonstrated via a luciferase reporter system to upregulate and downregulate the expression of
MaSBE2.3
, respectively.
...
PMID:Molecular identification of the key starch branching enzyme-encoding gene
SBE2.3
and its interacting transcription factors in banana fruits. 3263 29
