Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.4.1.14 (
SPS
)
813
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The cartilage lesion resulting from osteoarthritis (OA) always extends into subchondral bone. It is of great importance for simultaneous regeneration of two tissues of cartilage and subchondral bone. 3D-printed Sr
5
(PO
4
)
2
SiO
4
(
SPS
) bioactive ceramic scaffolds may achieve the aim of regenerating both of cartilage and subchondral bone. We hypothesized that strontium (Sr) and silicon (Si) ions released from
SPS
scaffolds play a crucial role in osteochondral defect reconstruction.
Methods:
SPS
bioactive ceramic scaffolds were fabricated by a 3D-printing method. The SEM and ICPAES were used to investigate the physicochemical properties of
SPS
scaffolds. The proliferation and maturation of rabbit chondrocytes stimulated by
SPS
bioactive ceramics were measured
in vitro
. The stimulatory effect of
SPS
scaffolds for cartilage and subchondral bone regeneration was investigated
in vivo
.
Results:
SPS
scaffolds significantly stimulated chondrocyte proliferation, and
SPS
extracts distinctly enhanced the maturation of chondrocytes and preserved chondrocytes from OA.
SPS
scaffolds markedly promoted the regeneration of osteochondral defects. The complex interface microstructure between cartilage and subchondral bone was obviously reconstructed. The underlying mechanism may be related to Sr and Si ions stimulating cartilage regeneration by activating HIF pathway and promoting subchondral bone reconstruction through activating Wnt pathway, as well as preserving chondrocytes from OA via inducing autophagy and inhibiting
hedgehog
pathway.
Conclusion:
Our findings suggest that
SPS
scaffolds can help osteochondral defect reconstruction and well reconstruct the complex interface between cartilage and subchondral bone, which represents a promising strategy for osteochondral defect regeneration.
...
PMID:Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface. 2955 66
