Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.3.1 (
citrate synthase
)
4,488
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Gel filtration chromatography showed that
nardilysin
activity in a rat testis or rat brain extract exhibited an apparent molecular weight of approximately 300 kDa compared to approximately 187 kDa for the purified enzyme. The addition of purified
nardilysin
to a rat brain extract, but not to an E. coli extract, produced the higher molecular species. The addition of a GST fusion protein containing the acidic domain of
nardilysin
eliminated the higher molecular weight
nardilysin
forms, suggesting that oligomerization involves the acidic domain of
nardilysin
. Using an immobilized
nardilysin
column, mitochondrial malate dehydrogenase (mMDH) and
citrate synthase
(CS) were isolated from a fractionated rat brain extract. Porcine mMDH, but not porcine cytosolic MDH, was shown to form a heterodimer with
nardilysin
. Mitochondrial MDH increased
nardilysin
activity about 50%, while
nardilysin
stabilized mMDH towards heat inactivation. CS was co-immunoprecipitated with mMDH only in the presence of
nardilysin
showing that
nardilysin
facilitates complex formation.
...
PMID:Nardilysin facilitates complex formation between mitochondrial malate dehydrogenase and citrate synthase. 1580 22
