Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.3.1 (
citrate synthase
)
4,488
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Tetrahymena
49kDa
protein functions as a
citrate synthase
(CS) and also assembles to 14-nm filament during cell mating. Bifunctional property of
49kDa
protein is suggested to be maintained by the difference of post-translational modification(s). We have found that phosphorylation is present on all three isoforms of
49kDa
protein. Dephosphorylation of
citrate synthase
type isoforms of
49kDa
protein, composing pl 7.7 and 8.0 isoforms, reduced its enzymatic activity, shifting these isoforms to basic side. In a course of dephosphorylation, isoform of pl 8.4 appeared with pl 7.7 and 8.0 isoforms, which correspond to the isoforms of 14-nm filament assembling type. With this dephosphorylation, the
citrate synthase
type isoforms obtained the ability to assemble 14-nm filaments. We propose that enzyme form and cytoskeletal form of
49kDa
protein were maintained simply by phosphorylation.
...
PMID:Enzymatic form and cytoskeletal form of bifunctional Tetrahymena 49kDa protein is regulated by phosphorylation. 1202 2
