Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.3.1 (
citrate synthase
)
4,488
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The interactions between pig heart
citrate synthase
and mitochondrial malate dehydrogenase or cytosolic malate dehydrogenase were studied using the frontal analysis method of gel filtration and by precipitation in polyethylene glycol. This method showed that an interaction between
citrate synthase
and mitochondrial malate dehydrogenase occurred but no interaction between
citrate synthase
and cytosolic malate dehydrogenase. Channeling of oxaloacetate in the malate dehydrogenase and
citrate synthase
-coupled systems was tested using polyethylene glycol precipitates of
citrate synthase
and mitochondrial malate dehydrogenase, and
citrate synthase
and cytosolic malate dehydrogenase. The effectiveness of large amounts of aspartate aminotransferase and
oxaloacetate decarboxylase
, as competing enzymes for the intermediate oxaloacetate, was examined. Aspartate aminotransferase and
oxaloacetate decarboxylase
were less effective competitors for oxaloacetate when precipitated
citrate synthase
and mitochondrial malate dehydrogenase in polyethylene glycol was used at low ionic strength compared with free enzymes in the absence of polyethylene glycol or with a co-precipitate of
citrate synthase
and cytosolic malate dehydrogenase. Substrate channeling of oxaloacetate with
citrate synthase
-mitochondrial malate dehydrogenase precipitate was inefficient at high ionic strength. These effects could be explained through electrostatic interactions of mitochondrial but not cytosolic malate dehydrogenase with
citrate synthase
.
...
PMID:Interaction between citrate synthase and malate dehydrogenase. Substrate channeling of oxaloacetate. 979 62
