Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.3.1 (
citrate synthase
)
4,488
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The pathogen
Botrytis cinerea
is a very dangerous pathogen that infects many economically important crops such as grape, strawberry, tomato, and eggplant. Cyprodinil, a pyrimidine amine fungicide, and fenhexamid, an amide fungicide, are new reagents for controlling gray mold with special efficacy. It is necessary to understand the change trends in the toxicological and physiological characteristics of
B. cinerea
with successive selective pressures of cyprodinil and fenhexamid to elongate the serving life of these fungicides for effective disease control. The toxicities of cyprodinil and fenhexamid at successive concentrations of EC
25
, EC
50
and EC
75
on
B. cinerea
strain BO5.10 were assayed along with mycelial growth-inhibition capacity. The results showed that the EC
50
value of the cyprodinil-treated F
27
strain increased approximately 18-fold, whereas of which in the fenhexamid-treated F
27
strain increased only 3-fold compared with that of the F
0
strain. The conductivities and glycerinum contents of the strains resistant to cyprodinil and fenhexamid were obviously enhanced; in contrast, the oxalic acid contents were decreased compared with those in the F
0
strain. The transcriptomes of the F
27
control (T01), cyprodinil-treated (T02) and fenhexamid- treated (T03) strains were analyzed, and the expression levels of functional genes in the T02 and T03 strains were significantly increased compared with those in the T01 strain; these results were further validated using qRT-PCR. The results indicated that the relative expression of two genes encoding mixed-functional oxidases (MFOs)
BC1G_16062
and
BC1G_16084
, two genes encoding transmembrane proteins
BC1G_12366
and
BC1G_13768
, two genes encoding Zinc finger proteins
BC1G_13764
and
BC1G_10483
,one gene encoding
citrate synthase
enzyme
BC1G_09151
, one gene encoding
gluconolactonase
BC1G_15612
in the T02 and T03 strains and one gene encoding lysophospholipids enzyme
BC1G_04893
in the T3 strain increased substantially compared with that in the T1 strain (
P
< 0.01). Functional prediction analysis of upregulated gene expression and structural verification was also performed, and the results showed that
BC1G_10483
was a ZnF_C2HC transcriptional regulator interacting with the Sp1 element of these genes to respond to the pressures from cyprodinil and fenhexamid. Our results could contribute to a better understanding of the resistance mechanism of
B. cinerea
against cyprodinil and fenhexamid.
...
PMID:Transcriptome and Resistance-Related Genes Analysis of
Botrytis cinerea
B05.10 Strain to Different Selective Pressures of Cyprodinil and Fenhexamid. 3042 1
