Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.3.1 (
citrate synthase
)
4,488
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Methane (CH
4
) is emerging as a candidate of signal molecule recently. However, whether or how CH
4
enhances plant adaptation to aluminum (Al)-contaminated environment is still unknown. In this report, the physiological roles and possible molecular mechanisms of CH
4
in the modulation of Al toxicity in alfalfa seedlings were characterized. Our results showed that, CH
4
pretreatment could alleviate Al-induced seedling growth inhibition and redox imbalance. The defensive effects of CH
4
against Al toxicity including the remission of Al-induced root elongation inhibition, nutrient disorder, and relative electrolyte leakage. Moreover, contents of organic acids, including citrate, malate, and oxalate, were increased by CH
4
. These results were paralleled by the findings of CH
4
regulated organic acids metabolism and transport genes,
citrate synthase
, malate dehydrogenase, aluminum-activated malate transporter, and aluminum activated citrate transporter. Consistently, Al accumulation in seedling roots was decreased after CH
4
treatment. In addition, Al-induced oxidative stress was also alleviated by CH
4
, through the regulation of the activities of anti-oxidative enzymes, such as
ascorbate peroxidase
, superoxide dismutase, and peroxidase, as well as their corresponding transcripts. Our data clearly suggested that CH
4
alleviates Al toxicity by reducing Al accumulation in organic acid-dependent fashion, and reestablishing redox homeostasis.
...
PMID:Methane enhances aluminum resistance in alfalfa seedlings by reducing aluminum accumulation and reestablishing redox homeostasis. 2881 65
