Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.3.1 (
citrate synthase
)
4,488
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The occurrence of Mediterranean fever with periods of increase and decrease has been recorded in the Crimean peninsula. The city of Sevastopol and its vicinity are known endemic areas for this disease. Some of the most active agents in the spread of this rickettsiosis are feral and abandoned dogs. The aim of this study was to test ticks parasitizing dogs in Sevastopol for the presence of
Rickettsia
using molecular methods. The testing of ticks was carried out using real-time PCR and the 'Real Best DNA Rickettsia species' kit (AO 'Vector-Best') followed by sequence identification of the rickettsial DNA detected. The
DNA marker
for
Rickettsia
species (a conservative area of
citrate synthase
gene,
glt
A) was detected in 16 of 84 (19.1%) samples of
Rhipicephalus sanguineus
ticks tested. Larger fragments of
glt
A,
omp
A and
sca
4 were amplified and sequenced for 10 of 16 PCR-positive samples.
Rickettsia
DNA amplified from eight of the samples matched the sequence of
Rickettsia conorii conorii
Malish, the causative agent of Mediterranean fever. The sequences of
Rickettsia
DNA from two other ticks had the closest match to homologous fragments of
Rickettsia massiliae
, a pathogenic spotted fever rickettsia that was identified in the Crimean Peninsula for the first time as part of this study. The detection of two pathogenic species of
Rickettsia
in the studied ticks suggests the potential for two rickettsial diseases in the region and warrants further epidemiological and clinical studies.
...
PMID:The role of
Rhipicephalus sanguineus
ticks parasitizing dogs in the spread of tick-borne rickettsial pathogens in the city of Sevastopol. 3257 90
