Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.3.3.1 (citrate synthase)
 4,488 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Predation is considered one of the main costs to reproduction but is rarely examined from a physiological perspective. In particular, little is known about the influence of brood predation pressure on the physiology of parents engaged in care. Brood defense, even when there is no direct threat to the parent, can be costly as it requires constant vigilance and chasing predators to protect the developing brood and maintain parental investment (i.e., fitness). Our goal was to examine the influence of natural variation in nest predation pressure on the physiology of the teleost smallmouth bass Micropterus dolomieu, an animal that provides sole-paternal care for developing offspring. More specifically, we used indicators of anaerobic (lactate dehydrogenase [LDH]) and aerobic capacity (cytochrome c oxidase [CCO] and citrate synthase [CS]) in axial white muscle and pectoral red muscle to test for differences in antipredator performance of nest guarding males across six lakes with natural variation in nest predation pressure. Pectoral red muscle enzyme activities and protein concentrations were highly conserved among populations, while axial white muscle showed differences in LDH activities, CCO activities and protein concentrations. However, there was no evidence for higher metabolic capacities in fish from lakes with increased brood predation pressure. Clearly, factors other than predation pressure have a greater influence on white muscle metabolic capacities. Additional research is needed to clarify the extent to which biotic and abiotic factors influence the enzyme activity and organismal performance in wild animals, particularly at the level of the individual and population.
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PMID:Brood predation pressure during parental care does not influence parental enzyme activities related to swimming activity in a teleost fish. 1985 86

The environmental persistence, bioaccumulative tendency and potential toxicity of perfluorooctane sulfonate (PFOS) have generated great concern. This study aimed at evaluating the toxicity of short-term PFOS exposure in gills of the European bullhead Cottus gobio, a candidate sentinel species, by monitoring the response of some enzymes (citrate synthase CS, cytochrome c oxidase CCO, and lactate dehydrogenase LDH), and by undertaking a proteomic analysis using 2D-DIGE. First, a 96-h exposure to 1mg PFOS/L significantly altered the activity of mitochondrial CS and CCO. Second, 2D-DIGE gels were used to compare gills from the control fish group with tissues from fish exposed for 96h to either 0.1 or 1mg PFOS/L. From the 27 protein spots displaying significant changes in abundance following PFOS exposure, a total of 20 different proteins were identified using nano LC-MS/MS and the Peptide and Protein Prophet of Scaffold software. The differentially expressed proteins that were identified are involved in the general stress response, ubiquitin-proteasome system, energy metabolism, and actin cytoskeleton, which provide clues on the cellular pathways and components mainly affected by PFOS. Moreover, our results showed that most proteins were differentially expressed at the low but not at the high PFOS concentration. This work provides insights into the biochemical and molecular events in PFOS-induced toxicity in gill tissue, and suggests that further studies on the identified proteins could provide crucial information to better understand the mechanisms of PFOS toxicity in fish.
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PMID:Ecotoxicoproteomics in gills of the sentinel fish species, Cottus gobio, exposed to perfluorooctane sulfonate (PFOS). 2139 90

The African cichlid, Pseudocrenilabrus multicolor victoriae is a eurytopic fish that exhibits high levels of developmental plasticity in response to dissolved oxygen availability. In this study, F1 offspring from three sites in the Mpanga River drainage of Western Uganda characterized by different dissolved oxygen (D.O.) regimes were reared under normoxic or hypoxic conditions. After 1 year, enzymes were measured to determine the tissue metabolic capacity of four different tissues: muscle, heart, brain and liver. The enzymes measured were pyruvate kinase [PK], lactate dehydrogenase [LDH], citrate synthase [CS], and cytochrome C oxidase [CCO], and an additional two, malate dehydrogenase (MDH) and fructose 1,6-bisphosphatase (FBPase), were examined in the liver only. Individuals reared under hypoxia exhibited elevated levels of LDH and CCO in the heart; and depressed activity levels of brain CS and liver CCO and MDH relative to normoxia-reared sibs. Results from this study demonstrate that long-term exposure to hypoxia during development can induce changes in the metabolic capacities of P. multicolor. This flexibility may be important in facilitating persistence in variable and/or novel environments, and in the face of increasing global hypoxia.
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PMID:Hypoxia-induced plasticity in the metabolic response of a widespread cichlid. 2397 8