Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.3.3.1 (citrate synthase)
 4,488 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The regulation of citrate-synthase (EC 4.1.3.7) synthesis in division-synchronized cultures of Euglena gracilis Klebs strain z was investigated. Citrate-synthase activity increased approximately two fold at the end of the light phase and in early dark phase in phototrophic cells synchronized by a 14 h light-10 h dark regime. Anti-(citrate synthase) was used to demonstrate that this increase in activity resulted from an increase in citrate-synthase protein. The amount of polyadenylated RNA per aliquot of culture increased midway through the light phase (before DNA replication) and had doubled by the end of this phase. Anti-(citrate synthase) was used to detect precursor citrate synthase in translations of total polyadenylated RNA in rabbit reticulocyte lysates. Citratesynthase mRNA was found to be present in cells at each stage of a division cycle, so that a stagespecific production of this mRNA to coincide with an increase in enzyme protein is unlikely. It is suggested that a post-transcriptional control operates in the regulation of citrate-synthase synthesis.
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PMID:Citrate-synthase mRNA in relation to enzyme synthesis in division-synchronized Euglena cultures. 2424 28

The effect of light and carbon nutrition on the synthesis of citrate synthase (EC 4.1.3.7) and malate dehydrogenase (EC 1.1.1.37) in dark-grown resting (carbon deficient) and in phototrophic division-synchronized cultures of Euglena gracilis Klebs strain z were investigated. Exposure of dark-grown Euglena to white or red light produced a transient increase in the specific activities of citrate synthase and malate dehydrogenase but blue light (of equal energy) was ineffective. Citrate-synthase activity increased at the end of the light phase and in early dark phase in phototrophic cultures division-synchronized by a regime of 14 h light-10 h dark. The addition of ethanol or malate produced a twofold increase in citrate-synthase activity compared with phototrophic cultures. White and blue light, but not red light, produced a transient repression of the metabolite-induced increase in citrate-synthase activity in division-synchronized cultures. Since only red light could effect a transient increase in the specific activity of mitochondrial enzymes, and the blue-red plastid receptor should respond to both blue and red light, the synthesis of mitochondrial enzymes in regreening cultures may be under the control of a new photoreceptor responding only to red light. In division-synchronized phototrophic cells the primary effector of synthesis of mitochondrial enzymes is not light but carbon nutrition.
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PMID:The regulation of synthesis of mitochondrial enzymes in regreening and division-synchronized Euglena cultures. 2425 32