Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
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Target Concepts:
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Query: EC:2.3.3.1 (
citrate synthase
)
4,488
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The bacterium
Pseudomonas syringae
is found in a variety of terrestrial and aquatic environments. Some strains of
P. syringae
express an ice nucleation protein (hereafter referred to as Ice+) allowing them to catalyze the heterogeneous freezing of water. Though
P. syringae
has been sampled intensively from freshwater sources in France, little is known about the genetic diversity of
P. syringae
in natural aquatic habitats in North America. We collected samples of freshwater from three different depths in Claytor Lake, Virginia, USA between November 2015 and June 2016. Samples were plated on non-selective medium (
TSA
) and on medium selective for
Pseudomonas
(KBC) and closely related species to estimate the total number of culturable bacteria and of
Pseudomonas
, respectively. A droplet freezing assay was used to screen colonies for the Ice+ phenotype. Ice+ colonies were then molecularly identified based on the
cts
(
citrate synthase
) gene and the 16S rDNA gene. Phylogenetic analysis of
cts
sequences showed a surprising diversity of phylogenetic subgroups of
P. syringae
. Frequencies of Ice+ isolates on
P. syringae
selective medium ranged from 0 to 15% per sample with the highest frequency being found in spring. Our work shows that freshwater lakes can be a significant reservoir of Ice+
P. syringae
. Future work is needed to determine the contribution of
P. syringae
from freshwater lakes to the
P. syringae
populations present in the atmosphere and on plants and, in particular, if freshwater lakes could be an inoculum source of
P. syringae
-caused plant disease outbreaks.
...
PMID:Diversity and Abundance of Ice Nucleating Strains of
Pseudomonas syringae
in a Freshwater Lake in Virginia, USA. 2833 77
