Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.3.1 (
citrate synthase
)
4,488
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Helicobacter pylori
has infected more than half of the world's population, causing
gastritis
, gastric ulcers, gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer. The oral recombinant
Helicobacter pylori
vaccine currently used has made great progress in addressing this problem, however, its efficacy and longevity still need to be improved. Th1 and Th17 cells play essential roles in local protection against
Helicobacter pylori
in the stomach mucosa. Additionally, protective immunodominant antigens are the preferred for a vaccine. In this work,
Helicobacter pylori
whole cell lysate was separated into 30 groups based on molecular weight by molecular sieve chromatography. The group best promoting CD4 T cells proliferation was selected and evaluated by immunization. The detail proteins were then analyzed by LC-MS/MS and expressed in Escherichia coli. Eleven proteins were selected and the dominant ones were demonstrated. As a result, three protective immunodominant antigens, inosine 5'-monophosphate dehydrogenase, type II
citrate synthase
, and urease subunit beta, were selected from
Helicobacter pylori
whole cell. Two of them (inosine 5'-monophosphate dehydrogenase and type II
citrate synthase
) were newly identified, and one (urease subunit beta) was confirmed as previously reported. The mixture of the three antigens showed satisfactory protective efficiency, with significant lower H. pylori colonization level (
P
< 0.001) and stronger Th1 (
P
< 0.001) and Th17 (
P
< 0.001) responses than PBS control group. Thus, inosine 5'-monophosphate dehydrogenase, type II
citrate synthase
, and urease subunit beta are three protective antigens inducing dominant Th1 and Th17 responses to defend against
Helicobacter pylori
infection.
...
PMID:Immunodominant antigens that induce Th1 and Th17 responses protect mice against
Helicobacter pylori
infection. 2955 92
