Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Expression of human immunodeficiency virus type 1 (HIV-1) can be activated in a chronically infected T-cell line (
ACH2
cells) by a cytokine, human tumor necrosis factor alpha (TNF-alpha). TNF-alpha treatment of
ACH2
cells resulted in an increase in steady-state levels of HIV RNA and HIV transcription. Gel mobility shift assays demonstrated that the transcriptional activation of the HIV long terminal repeat (LTR) by TNF-alpha was associated with the induction of a nuclear factor(s) binding to the NF-kappa B sites in the LTR. Deletion of the NF-kappa B sites from the LTR eliminated activation by TNF-alpha in T cells transfected with plasmids in which the HIV LTR directed the expression of the bacterial
chloramphenicol acetyltransferase
gene. Thus, TNF-alpha appears to activate HIV RNA and virus production by
ACH2
cells through the induction of transcription-activating factors that bind to the NF-kappa B sequences in the HIV LTR.
...
PMID:Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat. 276 7
