Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The binding of human immunodeficiency virus type 1 (HIV-1) gp120env to CD4 is the first event leading to infection and represents an important target for possible therapeutic intervention. To provide a tool for screening and quantitation of the effects of drugs inhibiting the Env-CD4 interaction, we developed a simple, fast and quantitative bioassay measuring the fusion between two cell lines generated by stable transfection: one expressing high levels of HIV-1 proteins but no infectious virus (
HL2
/3), and the other expressing the CD4 receptor and containing an inducible
chloramphenicol acetyltransferase
(
CAT
) gene linked to the HIV-1 long terminal repeat (HLCD4-
CAT
). Upon cocultivation of
HL2
/3 and HLCD4-
CAT
cells, efficient cell fusion is observed within 8 h. The efficiency of fusion can be evaluated visually and quantitated by measuring
CAT
enzyme. This novel bioassay allows testing for drugs capable of interfering with the CD4-Env interaction.
HL2
/3 cell line secretes gp120env in the medium and can be used for the production of Env protein.
...
PMID:A bioassay for HIV-1 based on Env-CD4 interaction. 207 9
