Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The proliferating cell nuclear antigen (PCNA) is an essential eukaryotic DNA replication factor that is transcriptionally regulated by the adenovirus oncoprotein E1A 243R. Inducibility of the human PCNA promoter by E1A 243R is conferred by the cis-acting PCNA E1A-responsive element (PERE), which associates with the ATF-1, cAMP response element-binding protein (CREB), and
RFX1
transcription factors and is modulated by cellular proteins such as the coactivator CREB-binding protein (CBP) and tumor suppressor p107 (Labrie, C., Lee, B. H., and Mathews, M. B. (1995) Nucleic Acids Res. 23, 3732-3741; Lee, B. H., and Mathews, M. B. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 4481-4486; Lee, B. H., Liu, M., and Mathews, M. B. (1998) J. Virol. 72, 1138-1145).
RFX1
also forms a complex with sequences in the PCNA promoter of mouse and rat that share homology with the
RFX1
consensus site. To explore the role of
RFX1
in regulating the PCNA promoter, we examined the effects of mutations in the human PERE on
RFX1
binding and gene expression. Mutations within the
RFX1
consensus binding site reduced
RFX1
binding, whereas mutations upstream of the site, or on its border, increased
RFX1
binding. These mutations also affected the transcriptional activity of PCNA-
chloramphenicol acetyltransferase
reporter constructs in transient expression assays. The relative transcriptional activity of mutant PCNA promoters, both in the presence and absence of E1A 243R, was inversely related to their ability to complex with
RFX1
. These findings suggest that the binding of
RFX1
is influenced by sequences outside its consensus binding site and that this transcription factor plays an inhibitory role in the regulation of PCNA gene expression.
...
PMID:Involvement of RFX1 protein in the regulation of the human proliferating cell nuclear antigen promoter. 1033 33
