Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Adipocyte
amino acid transporter
(
AAAT
) is induced during the 3T3-L1 preadipocyte differentiation process. In the -1819-bp 5'-upstream flanking region of the
AAAT
genomic gene, six DNase I protected sites were identified by using the 3T3-L1 adipocyte nuclear extract. Results of
chloramphenicol acetyltransferase
(
CAT
) expression from the chimeric
AAAT
promoter-driven
CAT
reporter gene indicated that one protein binding site, from -68 to -26, was essential for the promoter activity. However, this protein binding site does not contain recognition sites of the transcription factors important for adipocyte differentiation, i.e., the C/EBP or PPAR family. Further analysis revealed that the DNA sequence, TTCAAGTCCCGCCCTCCGCT from -65 to -46, was the cis-element essential and partially sufficient for inducible activity of the
AAAT
gene promoter.
...
PMID:An SP1-like cis-element is the major DNA motif for differential expression regulation of the adipocyte amino acid transporter. 1077 87
Trypanosoma brucei regulates gene expression by post-transcriptional mechanisms, such as mRNA turnover and translation control. This regulation frequently requires specific sequences located in the 3'-untranslated region. Microarray analysis and Northern blot hybridization showed that the
amino acid transporter
11 mRNA is up-regulated in insect stages of the parasite. By RT-PCR and sequencing, the AATP11 polyadenylation site was mapped. We show that this 3'-UTR causes higher expression of the
chloramphenicol acetyltransferase
(
CAT
) reporter gene in procyclic trypanosomes than in bloodstream forms. Results of deletion analysis suggested that multiple elements located between nucleotides 141 and 618 of the 3'-untranslated region are required for this control.
...
PMID:Regulation of an amino acid transporter mRNA in Trypanosoma brucei. 1799 63
