Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
DAN
gene was initially isolated as one of the genes whose expression is significantly decreased in a variety of transformed rat fibroblasts 3Y1 cells when compared with the parental 3Y1 cells. In the present study, we have isolated the genomic clone of the
DAN
gene from a 3Y1 genomic library and characterized the possible regulatory elements responsible for the transcription of the
DAN
gene. The transcription initiation site was determined by a primer extension experiment. Putative TATA and CAAT-like elements were present 31 and 358 bp upstream from the transcription start site, respectively. Transient transfection of a series of
DAN
-
chloramphenicol acetyltransferase
(
CAT
) gene constructs, which contain different portions of the 5'-flanking region (2,236 bp) of the
DAN
gene and the
CAT
gene, was used to localize a regulatory element. These experiments demonstrated the presence of the regions that regulate
DAN
gene expression positively (-57 to +118) and negatively (-1,232 to -636). The electrophoretic mobility-shift assays revealed that 3Y1 and SR-3Y1 nuclear extracts specifically interact with the positive (-57 to +118) and the negative (-1,226 to -987) regulatory regions, respectively.
...
PMID:Identification of essential cis-acting regulatory elements for transcription of the rat DAN gene. 921 71
