Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have examined the chicken cytoskeletal actin gene for promoter activity and for the presence of cis-acting gene transcription activating sequences. Plasmids were constructed with the beta-actin promoter or other fragments of the beta-actin gene adjacent to either the truncated Herpes simplex virus (
HSK
)-tk promoter driving the neo gene or the enhancerless simian virus 40 (SV40) promoter driving the
chloramphenicol acetyltransferase
(
CAT
) gene. The neo plasmids were tested for frequency of transformation of mammalian cells to G418 resistance. The
CAT
constructions were tested for
CAT
expression in both transient and stable expression systems. We find that the beta-actin promoter is very strong in all of the assay systems and is as strong as any promoter we have tested. Also, we find that there are sequences in the vicinity of the beta-actin promoter which act like an enhancer sequence in activating transcription from the truncated HSV-tk promoter and the enhancerless SV40 promoter. Constructs with these actin sequences augment the transformation frequency of the neo plasmids, and stimulate the level of
CAT
expression from the
CAT
plasmids after stable chromosome insertion but not during the transient expression phase.
...
PMID:Activating elements in the promoter region of the chicken beta-actin gene. 347 Feb 37
