Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The muscle-specific isoform of
phosphofructokinase
(
PFK-A
) is induced during muscle development. To understand expression of
PFK
at the molecular level, transcription of the mouse
PFK-A
gene was examined during C2 myoblast differentiation to myotubes.
PFK-A
gene transcription increased 5-7-fold during differentiation in vitro. To identify cis-acting elements which direct muscle-specific transcription of the
PFK-A
gene, its 5'-flanking region and first exon were cloned and characterized. S1 nuclease protection and primer extension assays showed four sites of transcription initiation at 106, 105, 88, and 87 bp upstream of the translation initiation codon. Stable transfection of fusion genes linking -1900 to +99 of
PFK-A
5'-flanking sequence to
chloramphenicol acetyltransferase
coding sequences into myogenic C2 cells did not confer muscle-specific expression. However, larger fragments of
PFK-A
5'-flanking region (-5800 to +99) showed muscle-specific expression by transient transfection assay. The sequences directing muscle-specific transcription were further defined by linking various
PFK-A
upstream fragments to the luciferase gene under the control of the
PFK-A
proximal promoter, -335 to +99 bp. We found DNA sequence responsible for muscle-specific expression of the
PFK-A
gene between -4800 and -3900 bp.
...
PMID:Control of the murine phosphofructokinase-A gene during muscle differentiation. 811 Jul 79
