Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.3.1.28 (chloramphenicol acetyltransferase)
 5,100 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The in vivo promoter activity of the 5'-region of the genes encoding the carotenogenic enzymes phytoene desaturase (crtP) and phytoene synthase (crtB) from the cyanobacterium Synechocystis sp. PCC 6803 has been studied by deletion mapping, primer extension, Northern blot, and using the chloramphenicol acetyltransferase (cat) reporter gene. crtP and crtB are closely linked in the Synechocystis genome, but it is shown that both genes are independently transcribed. The level of transcription in both cases is very low. Their expression is affected in a similar way by light intensity: very little or no expression is observed in the dark compared with expression under illumination conditions. High light intensities induce a transient increase in transcription initiation as detected with the cat probe. DNA fragments containing 81 base pairs upstream of the crtP putative transcription initiation site or 192 base pairs upstream of the crtB putative transcription initiation site are enough to confer light sensitive expression to a reporter gene.
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PMID:Characterization of two carotenoid gene promoters in the cyanobacterium Synechocystis sp. PCC 6803. 987 19

We have developed near-isogenic introgression lines (NIILs) of an elite indica rice cultivar (IR64) with the genes for beta-carotene biosynthesis from dihaploid (DH) derivatives of golden japonica rice (cv. T309). A careful analysis of the DH lines indicated the integration of the genes of interest [phytoene synthase (psy) and phytoene desaturase (crtI)] and the selectable marker gene (hygromycin phosphotransferase, hph) in two unlinked loci. During subsequent crossing, progenies could be obtained carrying only the locus with psy and crtI, which was segregated independently from the locus containing the hph gene during meiotic segregation. The NIILs (BC(2)F(2)) showed maximum similarity with the recurrent parent cultivar IR64. Further, progenies of two NIILs were devoid of any fragments beyond the left or right border, including the chloramphenicol acetyltransferase (cat) antibiotic resistance gene of the transformation vector. Spectrophotometric readings showed the accumulation of up to 1.06 microg total carotenoids, including beta-carotene, in 1 g of the endosperm. The accumulation of beta-carotene was also evident from the clearly visible yellow colour of the polished seeds.
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PMID:Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of 'golden' indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue. 1717 11