Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A plasmid expression vector was constructed to direct the synthesis of foreign proteins in Escherichia coli as fusions with
cyclomaltodextrin glucanotransferase
(
CGT
) with cytoplasmic location (delta ssCGT). The ability of
CGT
to bind to covalently immobilized cyclodextrins was utilized in purifying fused target proteins. A large proportion of the cytoplasmically synthesized delta ssCGT formed inclusion bodies which adopted the active conformation at considerably high refolding concentration (67 microM delta ssCGT solution). By lowering the cultivation temperature the proportion of the soluble delta ssCGT was slightly increased. Intracellularly expressed delta ssCGT provides a potential affinity handle which forms easily refoldable inclusion bodies increasing the yield and stability, and possibly allows the expression of lethal target proteins. Interestingly, the interaction between one model fusion protein delta ssCGT-CAT (CAT,
chloramphenicol acetyltransferase
) and the E. coli heat shock protein GroEL was observed.
...
PMID:Expression in E. coli and purification of intracellular proteins by fusion to cyclomaltodextrin glucanotransferase. 136 55
