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Query: EC:2.3.1.28 (chloramphenicol acetyltransferase)
 5,100 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An operon fusion was constructed in which the chloramphenicol acetyltransferase gene (cat) is under the transcriptional control of the anaerobically-activated formate dehydrogenase (fdhF) gene promoter. It was used as a screening system for mutations in trans which prevent the formate-dependent anaerobic induction of fdhF gene expression. Five classes of mutants were identified. The defect in class I mutants was complemented by a plasmid (pBA11) or subclones thereof, which harbor genes of the Escherichia coli 58 min hyd (hydrogenase) gene cluster. They may comprise regulatory gene mutants. The phenotype of class II mutants was reversed by supplementing the medium with 100 microM MoO4(2-); WO4(2-) could substitute for MoO4(2-) in restoring anaerobic induction by formate. Similarly, class III mutants were phenotypically suppressed by inclusion of 500 microM Ni2+ in the medium; these mutants were shown to carry a defective fnr gene. The mutant of class IV had a defect in a formate dehydrogenase structural gene and that of class V was unable to grow under fermentative conditions while maintaining the capability to grow anaerobically in the presence of electron acceptors.
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PMID:Mutations in trans which affect the anaerobic expression of a formate dehydrogenase (fdhF) structural gene. 266 74

The Clostridium pasteurianum hydrogenase I has been expressed in the cyanobacterium Synechococcus PCC7942. The Shine-Dalgarno sequence of the structural gene encoding hydrogenase I from C. pasteurianum was changed to that of the cat (chloramphenicol acetyltransferase) gene. The hydrogenase gene was cloned downstream of a strong promoter, isolated from Synechococcus PCC7942, with the cat gene as a reporter gene. Expression of clostridial hydrogenase was confirmed by Western and Northern blot analyses in Synechococcus and Escherichia coli, whereas in vivo/in vitro measurements and activity staining of soluble proteins separated on non-denaturing polyacrylamide gels revealed functional expression of hydrogenase only in cyanobacterial cells. The changed Shine-Dalgarno sequence appeared to be essential for the functional expression of clostridial hydrogenase in Synechococcus, but had no influence on the expression and activity of clostridial hydrogenase expressed in E. coli.
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PMID:Heterologous expression of clostridial hydrogenase in the Cyanobacterium synechococcus PCC7942. 1068 72