Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
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Target Concepts:
Gene/Protein
Disease
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Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The vascular cell adhesion molecule 1 (VCAM-1) is a 110-kD member of the immunoglobulin gene superfamily expressed on the surface of interleukin 1 beta- or tumor necrosis factor alpha (TNF)-stimulated endothelial cells. The cell surface protein functions as an inducible adhesion receptor for circulating mononuclear leukocytes and some tumor cells. We have previously characterized the genomic organization of the
VCAM1
gene and described its chromosomal localization. In this report, the promoter of the
VCAM1
gene is characterized. New transcription of the
VCAM1
gene occurred when endothelial cells were treated with TNF. Fusion plasmids containing the 5' flanking sequence of the
VCAM1
gene and the
chloramphenicol acetyltransferase
reporter gene were used to identify cis-acting sequences that direct the cytokine-induced transcription. When transfected into bovine aortic endothelial cells, constructs containing 755 bp of the 5' flanking sequence were induced by TNF. Within the cytokine-responsive region of the core promoter were functional NF-kappa B and GATA elements. Upstream of the core promoter, the
VCAM1
5' flanking sequence contained a negative regulatory activity. NF-kappa B-mediated activation of
VCAM1
gene expression may lead to endothelial expression of a mononuclear leukocyte adhesion molecule associated with initial events in the development of an atherosclerotic lesion.
...
PMID:Functional analysis of the human vascular cell adhesion molecule 1 promoter. 128 Dec 11
