Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:2.3.1.28 (
chloramphenicol acetyltransferase
)
5,100
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have developed six transgenic lines of mice with constructs containing presumptive 5' regulatory regions of carbonic anhydrase II (
CA II
). Four of the lines contained 1,100 bases of the 5' flanking region of the human
CA II
gene, and two transgenic lines resulted from a construct containing 500 bases of the 5' flanking region of the mouse
CA II
gene. Tissue-specific expression of the
chloramphenicol acetyltransferase
(
CAT
) gene was not obtained in any of the transgenic lines. One of the transgenic lines was found to have high levels of expression of
CAT
in cerebellum. This expression persisted through multiple generations and was independent of the parental origin of the transgene. On the assumption that the expression was due to the insertion of the transgene in or near a gene expressed normally in cerebellum, homozygous mice were bred for the transgenic insert to see if a mutation might have been induced. Homozygous mice were found and seemed to be normal in all aspects of their phenotype studied. Thus, in this case, neither the insertion of the gene nor the ectopic expression of
CAT
seemed to be harmful to the animals.
...
PMID:Ectopic expression of chloramphenicol acetyltransferase (CAT) in the cerebellum in mice transgenic for a carbonic anhydrase II promoter-CAT construct that is without apparent phenotypic effect. 212 91
The upstream promoter sequences of the human carbonic anhydrase II (
CA II
) gene have been studied by 5' deletion analysis. Promoter activity was assayed by transfection and
chloramphenicol acetyltransferase
assay in both human HeLa cells and murine L cells. This investigation showed that the
CA II
promoter is comparable in activity to that of the simian virus 40 early-region promoter and enhancer and that the
CA II
upstream sequences exert a different pattern of control in the two cell lines.
...
PMID:Molecular analysis of G+C-rich upstream sequences regulating transcription of the human carbonic anhydrase II gene. 283 May
