Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.21 (
CPT
)
4,580
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have previously shown that metoprolol can inhibit
carnitine palmitoyltransferase
-1 catalytic activity and decrease its malonyl CoA sensitivity within 30 min, suggesting the importance of a covalent modification. The aim of this study was to characterize the effects of PTMs on
CPT
-1 in the heart. Mitochondria were isolated from the hearts of male Wistar rats and incubated with kinases of interest (protein kinase A,
CAMK-II
, p38 MAPK, Akt) or with peroxynitrite and sodium nitroprusside. PKA decreased
CPT
-1 malonyl CoA sensitivity, associated with phosphorylation of
CPT
-1A, whereas
CAMK-II
increased malonyl CoA sensitivity by phosphorylating
CPT
-1B. p38 bound to
CPT
-1B and stimulated
CPT
-1 activity. The association of
CPT
-1 with these kinases and their scaffolding proteins was confirmed in co-localization studies. Peroxynitrite and sodium nitroprusside reversibly stimulated
CPT
-1 activity, and the change in
CPT
-1B activity was most consistently associated with glutathiolation of
CPT
-1B. These studies have identified a new regulatory system of kinases, scaffolding proteins and thiol redox chemistry which can control cardiac
CPT
-1 in vitro.
...
PMID:Functional effects of protein kinases and peroxynitrite on cardiac carnitine palmitoyltransferase-1 in isolated mitochondria. 1986 3
