Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.3.1.21 (
CPT
)
4,580
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The Chinese mitten crab (
Eriocheir sinensis
) is a commercially important crab in China and is usually managed at high stocking densities. Agonistic behavior directly impacts crab integrity, survival, and growth and results in economic losses. In the present study, we evaluated the modulatory effects of serotonin (5-HT) and dopamine (DA) though the 5-HT2 and DA2 receptor-cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) pathway on agonistic behavior. The results showed that injection of either 10
-6
mol/crab 5-HT or DA reduced the agonistic behavior of
E. sinensis
(
P
< 0.05), as did 10
-10
mol/crab DA and 10
-8
mol/crab 5-HT and DA (
P
< 0.05); however, a dose of 10
-10
mol/crab 5-HT promoted agonistic behavior. 5-HT significantly increased the mRNA expression level of
5-HT7
receptor and reduced that of the DA2 receptor in the cerebral ganglion (
P
< 0.05). In contrast to 5-HT, DA significantly decreased 5-HT2B mRNA levels and increased
5-HT7
and DA2 receptor levels in the thoracic ganglia (
P
< 0.05). In addition, injections of either 5-HT or DA increased the cAMP and PKA levels in hemolymph (
P
< 0.05). By using in vitro culture of the thoracic ganglia, the current study showed that ketanserin (5-HT2 antagonist) and [R(-)-TNPA] (DA2 agonist) had obvious effects on the expression levels of the two receptors (
P
< 0.05). In vivo experiments further demonstrated that ketanserin and [R(-)-TNPA] could both significantly reduce the agonistic behavior of the crabs (
P
< 0.05). Furthermore, both ketanserin and [R(-)-TNPA] promoted the cAMP and PKA levels (
P
< 0.05). The injection of
CPT
-cAMP (cAMP analogue) elevated the PKA levels and inhibited agonistic behavior. In summary, this study showed that 5HT-2B and DA2 receptors were involved in the agonistic behavior that 5-HT/DA induced through the cAMP-PKA pathway in
E. sinensis
.
...
PMID:5-HT2B, 5-HT7, and DA2 Receptors Mediate the Effects of 5-HT and DA on Agonistic Behavior of the Chinese Mitten Crab (
Eriocheir sinensis
). 3164 70
